College of Pharmacy, Guangxi University for Chinese medicine, No.13 , Wu He street, Qingxiu District, Nanning ,530200 , Guangxi Province, China
Publications
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Research Article
A Novel Nitidine Chloride
Nanoparticle Inhibits the Stemness of CD133+EPCAM+ Huh7 Hepatocellular
Carcinoma Cells
Author(s): Danni Li, Qiying Zhang, Yuzhu Zhou, Hua Zhu* and Tong Li*
Abstract
Nitidine chloride is a natural product. We synthesized novel nanoparticles of
Nitidine chloride (TPGS-FA/NC), which evaluated anti-hepatocellular carcinoma
potential capability in vitro and in vivo. Cell viability was assessed by MTT and
colony assays; TPGS-FA/NC was examined by Confocal Microscopy though
targeting Huh7 Hepatocellular Carcinoma Cells. A sphere culture technique was
used to enrich Cancer Stem Cells (CSC) in Huh7 cells. The in vivo antitumor
efficacy of TPGS-FA/NC was evaluated in Huh7 cell xenograft model, which were
administered by TPGS-FA/NC for 2 weeks. TPGS-FA/NC (10, 20, 40 μg/mL)
dose-dependently inhibited the proliferation of HCC cells. Interestingly, TPGS-
FA/NC (10, 20, 40 μg/mL) drastically reduced the EpCAM+/CD133+ cell numbers,
which suppressed the sphere formation and inhi..
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